Cholesterol Oxidation And Its Applications Advances In Microbiology

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Cholesterol oxidase (EC 1.1.3.6, ChoA) is a FAD-dependent bifunctional enzyme that catalyzes the oxidation and isomerization of cholesterol (Figure 1, 1) to cholest-4-en-3-one (3) while dioxygen is finally reduced to H 2 O 2 as by-product. The enzymatic overall cholesterol oxidation comprises three steps.

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